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When I cloned PCR products in pJET I plate something like 25-50 µl of the I have used the pJet cloning kit and according to the instruction prepared the . We are using pJET1.2 vector (Thermo CLoneJET PCR Cloning kit) for cloning 1400bp fragment. . But when I calculate by manually or by online it shows different. All components of the kit were function tested in control experiment as described in the manual. A 2 l aliquot of the ligation mixture was used to transform 50 l of 8 Dec 2014 "This PCR Cloning Kit contains an optimized 2X Cloning Master Mix with a proprietary ligation enhancer and a It allows simple and quick cloning of any PCR amplicon, whether the amplification reactions are Protocols, Manuals & Usage. All components of the Thermo Scientific™ CloneJET™ PCR Cloning Kit should be stored at -20°C. described in the manual. A 2 L aliquot of the ligation Efficiently clone PCR products generated with any thermostable DNA polymerase using Thermo Scientific™ CloneJET™ PCR Cloning Kit, a positive selection CERTIFICATE OF ANALYSIS. All components of the kit were function tested in control experiment as described in the manual. A 2 µl aliquot of the ligation Thermo Scientific CloneJET PCR Cloning Kit is an advanced positive selection system for high-efficiency cloning of PCR products generated with any thermostable DNA polymerase. Blunt-ended PCR products generated with a proofreading enzyme are ligated directly into the cloning Available with the CloneJETT" PCR Cloning Kit (#K1231, #K1232). pJET1.2/blunt vector map. DNA sequence of MCS region. Bpil. 58. 892. 417. Enzymes which CloneJet PCR Cloning Kit. 2015-01-13 CloneJet PCR Cloning Kit product from ThermoScientific original manual can be found here